Composite

Part:BBa_K4737020:Design

Designed by: Peisong Wu   Group: iGEM23_BNUZH-China   (2023-10-04)


prpsM::T7 RNA Polymerase-T7 Promoter::GFP-shRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal PstI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal PstI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal PstI site found at 34
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1985


Design Notes

None.


Source

The BNUZH-China iGEM team 2023 designed and built.

References

Fu L, Xu J, Han X, Yang H, Lai Y, Huang Z, Gong J. [Construction and evaluation of a pUC-type prokaryotic promoter reporter system based on lacZ gene]. Sheng Wu Gong Cheng Xue Bao. 2021 Jan 25;37(1):321-330. Chinese. doi: 10.13345/j.cjb.200262. PMID: 33501812.